Composite

Part:BBa_K952007:Design

Designed by: Saimon Sharif   Group: iGEM12_Columbia-Cooper-NYC   (2012-10-01)


IPTG induc. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 970
    Illegal NgoMIV site found at 1482
    Illegal NgoMIV site found at 1775
    Illegal NgoMIV site found at 1869
    Illegal AgeI site found at 504
    Illegal AgeI site found at 1650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1539
    Illegal BsaI.rc site found at 403


Design Notes

N/A


Source

Part BBa_K952002 was a composite part designed by our team, see BBa_K952002 for further information. BBa_J04500 was obtained from the 2012 kit.

References